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S0149
Sigma
Streptolysin O from Streptococcus pyogenes
γ-irradiated
| CAS Number: | 98072-47-0 |
| EC Number: | 308-500-3 |
| MDL number: | MFCD00132389 |
Description
| Application | Permeabilizes membranes to permit cellular uptake of large or charged molecules. |
| Preparation Note | Prepared from S 5265 |
| Unit Definition | One unit will cause 50% lysis of 2% red blood cell suspensions in phosphate buffered saline, pH 7.4, after incubation at 37 °C for 30 min. |
| Biochem/physiol Actions | Thiol-activated toxin that permeabilizes animal cell membranes. The protein binds as a monomer to membrane cholesterol and subsequently polymerizes into large arc- and ring-shaped structures surrounding pores of >12 nm. |
Properties
| sterility | γ-irradiated |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| reference | Alouf, J.E. Pharmacol. Ther. 11, 661, (1980) |
| Bhakdi, S., et al. Infect. Immun. 46, 394, (1984) | |
| Wagner, A.C. and Williams, J.A. Pancreas 11, 236-240, (1995) | |
| Raufman, J.P., et al. Biochim. Biophys. Acta 1357, 73-80, (1997) | |
| Palmer, M., et al. Biochemistry 37, 2378-2383, (1998) |
